Transcriptome profiling of ripening nectarine (Prunus persica L. Batsch) fruit treated with 1-MCP
نویسندگان
چکیده
A large-scale transcriptome analysis has been conducted using microPEACH1.0 microarray on nectarine (Prunus persica L. Batsch) fruit treated with 1-methylcyclopropene (1-MCP). 1-MCP maintained flesh firmness but did not block ethylene biosynthesis. Compared with samples at harvest, only nine genes appeared to be differentially expressed when fruit were sampled immediately after treatment, while a total of 90 targets were up- or down-regulated in untreated fruit. The effect of 1-MCP was confirmed by a direct comparison of transcript profiles in treated and untreated fruit after 24 h of incubation with 106 targets differentially expressed. About 30% of these targets correspond to genes involved in primary metabolism and response processes related to ethylene, auxin, and other hormones. In treated fruit, altered transcript accumulation was detected for some genes with a role in ripening-related events such as softening, colour development, and sugar metabolism. A rapid decrease in flesh firmness and an increase in ethylene production were observed in treated fruit maintained for 48 h in air at 20 degrees C after the end of the incubation period. Microarray comparison of this sample with untreated fruit 24 h after harvest revealed that about 45% of the genes affected by 1-MCP at the end of the incubation period changed their expression during the following 48 h in air. Among these genes, an ethylene receptor (ETR2) and three ethylene-responsive factors (ERF) were present, together with other transcription factors and ethylene-dependent genes involved in quality parameter changes.
منابع مشابه
Jasmonate-induced transcriptional changes suggest a negative interference with the ripening syndrome in peach fruit.
Peach (Prunus persica L. Batsch) was chosen as a model to shed light on the physiological role of jasmonates (JAs) during fruit ripening. To this aim, the effects of methyl jasmonate (MJ, 0.40 mM) and propyl dihydrojasmonate (PDJ, 0.22 mM), applied in planta at different fruit developmental stages, on the time-course of ethylene production and fruit quality traits were evaluated. MJ-induced cha...
متن کاملPhenotypic diversity among local Spanish and foreign peach and 1 nectarine [ Prunus persica ( L . ) Batsch ] accessions
متن کامل
First genetic linkage map of chilling injury susceptibility in peach (Prunus persica (L.) Batsch) fruit with SSR and SNP markers
Peach and nectarine (Prunus persica L) are highly perishable; they ripen and deteriorate quickly at ambient temperature. Storage at low temperature (0–5oC) is a common strategy used to slow the ripening processes and extending shelf life. However, if susceptible varieties are held too long at a low temperature, they will not ripen properly and will develop chilling injury (CI) symptoms like mea...
متن کاملJASMONATES NEGATIVELY INTERFERE WITH FRUIT DEVELOPMENT AND RIPENING IN PEACH (Prunus persica L. Batsch)
Jasmonates (JAs) are signal molecules which elicit stress responses through JAs-responsive genes; they are also involved in developmental processes, including fruit growth and ripening. In the present study, methyl jasmonate (MJ) and n-propyl dyhydrojasmonate (PDJ) were applied to peach trees at the S1, S3 and S4 phases. In all cases, both chemicals inhibited climacteric ethylene production and...
متن کاملAssociation genetics of chilling injury susceptibility in peach (Prunus persica (L.) Batsch) across multiple years
Peach and nectarine (Prunus persica L.) are highly perishable; they ripen and deteriorate quickly at ambient temperature. Storage at low temperature (0-5 °C) is a common strategy used to slow the ripening processes and to extend shelf life. However, if susceptible varieties are held too long at a low temperature, they will not ripen properly and will develop chilling injury (CI) symptoms like m...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of Experimental Botany
دوره 59 شماره
صفحات -
تاریخ انتشار 2008